Total Dietary Fiber Assay Kit

sufficient for ~100 assays

Category

PROPIEDADES

uso

sufficient for ~100 assays

Nivel de calidad

100

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

temp. de almacenamiento

2-8°C


Aplicación

This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinized with heat stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analyzed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash.

Otras notas

Reagents for the determination of total dietary fiber.

Principio

Uses a combination of enzymatic and gravimetric methods. This procedure is based on the method published in Official Methods of Analysis, 15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990).

Solo componentes del kit

Referencia del producto
Descripción

    • α-Amylase, Heat-stable 10 mL

    • Amyloglucosidase 10 mL

    • Celite 50 g

  • Protease 500 mg